This Dionex Summit HPLC System includes:
- SOR-100 Solvent Degasser
- P680A HPG-2 High-Pressure Gradient Pump with 2 eluent lines
- ASI-100 Automated Sample injector with100-μL syringe
- TCC-100 Thermostatted Column Compartment
- UVD170U UV/Vis Detector
- PC with software
Other HPLC components are available if you require a different configuration.
P680 HPLC Pump
| Specification | |
| Flow rate range | 1-10,000 µL/min |
| Flow rate accuracy and reproducibility | ±0.1% at 1 mL/min |
| Pressure range | 0.1–50 MPa (7250 psi) |
| Pressure ripple | Typically <1% |
| Proportioning accuracy | ±0.5% at 2 mL/min |
| Proportioning reproducibility | ±0.5% at 2 mL/min |
Dionex ASI-100 Automated Sample injector
| Specification | |
| Injection principle | In-line split-loop |
| Sample capacity | Depending on the vial carrier; carrier with:
3 anal./cylind. segments = 117 vials at 1.8 ml + 3 at 4 ml 3 anal./concial segments = 117 vials at 1.1 ml + 3 at 4 ml 3 mini segments = 192 vials at 1.2 ml + 3 at 4 ml 3 mini segments/Beckmann = 192 vials at 0.25ml + 3 at 4 ml 3 semiprep. segments = 63 vials at 4 ml + 3 at 4 ml 3 Eppendorf segments = 66 vials at 2.0 ml + 3 at 4 ml 3 Eppendorf/concial segments = 66 vials at 1.5 ml + 3 at 4 ml 3 Eppendorf/conical segments = 66 vials at 0.5 ml + 3 at 4 ml |
| Vial types | Depending on the segment type:
Type 01 (Analyt./Cylindrical): 1.8 ml vials, cylindrical, with crimp cap Type 01 (Analyt./Conical): 1.1 ml vials, conical, with crimp cap Type 02 (Mini): 1.2 ml vials, cylindrical, with crimp cap Type 03 (Semiprep): 4 ml vials, cylindrical, with screw cap All above vials with silicone/PTFE septa. Type 02 (Mini/Beckmann): 0,25 ml Beckmann mini vials (Beckmann part no. 652823) Type 04 (Eppendorf): 2.0 ml Eppendorf vials (Eppendorf part no. 0030 120.094) Type 04 (Eppendorf/Conical): 1.5 ml Eppendorf vials, conical (Eppendorf part no. 0030 120.086) Type 04 (Eppendorf/Conical): 0.5 ml Eppendorf vials, conical (Eppendorf part no. 0030 121.023) |
| Injection Volume | ASI-100/ASI-100T: 1 – 250 µl in 0.1µl increments
ASI-100P/ASI-100PT: 10 – 2500 µl in 1 µl increments |
| Carry-Over | < 0.1% |
| Linearity | RSD < 0.5 % |
| Flush volume | None |
| Sample loss/injection | None |
| Min. required sample | 5µl (ASI-100/ASI-100T) |
| Reproducibility | Specified RSD < 0.3% at 5µl (250 µl syringe)
Typical RSD < 0.2% at 5 µl 250 µl syringe) Typical RSD < 1.0% at 1µl 250 µl syringe) Specified RSD < 0,3% at 100 µL (2500 µl syringe) |
| Repeat injections | Max. 9 per vial |
| Counters | For a description of the various counters that provide information about the autosampler status |
| Method Parameters | For a detailed description of the method parameters |
| Safety features | Overflow/leak sensor, automatic segment detection (when enabled), tray detection/manual interference monitoring (when enabled), automatic vial recognition (i.e., monitoring in case no vial is present at the specified position), electronic needle protection, all mechanical operations internally monitored (injection valve, syringe etc.) |
| Temperature Control
(ASI-100T/ ASI-100PT) |
In the range of 4 – 45°C (39.2 – 113°F)
cooling: -18°C (32.4°F) from ambient heating: +35°C (63°F) from ambient |
| Input/Display | LCD, keypad with control elements and function keys for direct control, LED display (7 segments) for retention time indication, LED display (7 segments) plus three LEDs (red/green/blue)
indicating the sample position, 2 LEDs (Load/Inject) indicating the status of the motorized switching valve, 2 LEDs (cool/heat) indicating the temperature status (ASI-100T/ASI-100PT) |
| Wetted parts | Stainless steel (1.4571/316SS), PCTFE, PTFE, PEEK, Vespel |
| Operating pressure | Max. 340 bar (34 MPa) Max 200 bar (20 MPa) with 2.5 ml syringe + corresponding sample loop |
| In-/Outputs | 2x serial (RS232[A] and RS232 [B]), MSV, Sample Position, Digital I/O |
Dionex TCC-100 Thermostatted Column Compartment
| Specification | |
| Environmental
Conditions |
Range of use: Indoor use
Temperature: 10°C to 35°C (50°F to 95°F) Air humidity: 40 to 85% rel. humidity, non-condensing Overvoltage Category: II Polution degree: 2 |
| Operating Conditions | Temperature range: +5°C to +85°C (+41°F to +185°F)
When cooling: max. 15°K from ambient |
| Temperature Setting | In 0.1°C increments |
| Temperature Accuracy | ± 0.5°C |
| Temperature Stability | ± 0.1°C |
| Temperature Precision | ± 0.1°C |
| Heat-up/Cool-down Time | Less than 20 minutes from 20°C to 50°C
Less than 20 minutes from 50°C to 20°C average change per minute: 2-4°K |
| Column Capacity | 3 columns (maximum length: 30 cm) |
| Control | All functions controllable via LAN or USB
Permanent status transfer Program-controlled by Chromeleon or stand-alone operation |
| Switching Valve | Options: 2-position/6-port or 2-position/10-port switching valve
Maximum backpressure: 345 bar (5000 psi) Switching time: 110 ms |
| GLP | When controlled by Chromeleon:
Dionex AutoQ routines for Instrument Qualification Column identification system for 2 columns |
