The 1260 Infinity Autosampler is part of the 1260 Infinity LC system that is compatible with operating pressures up to 1200 bar. It features the next-generation flow-through design for high precision injection of both large and small volumes without the need to change sample loops. Only the injected volume is required from the sample and no valuable sample is wasted for flushing. Inert materials in the metering device seal and needle seat as well as reduced hydraulic volumes achieve extremely low carryover. A new module, the 1260 Infinity Flexible Cube, combines seamlessly with the autosampler for infinite injection flexibility. The Flexible Cube facilitates fixed-loop injection mode for ultra-fast cycle times as well as automatic backflushing of the autosampler’s needle seat for lowest carryover yet.
Features:
- Next generation flow-through design with ultra low system volume for operation up to 1200 bar
- Highest injection precision for both large and small volumes without changing loops
- No waste of valuable sample for flushing
- Short injection cycles for higher productivity through overlapped injections
- Best carryover performance through inert seal and needle seat material as well as backflush of needle seat (requires 1290 Infinity Flexible Cube)
- New cartridge loop for ultimate ease-of-use
- Flexible and convenient sample handling with different types of sample containers such as well-plates and vials – process up to 768 samples unattended with 384-well plates
- Peltier temperature control from 4 to 40 °C for thermally labile samples (requires 1290 Infinity Thermostat)
Specifications:
Injection range | >0.01 – 8 µL in 0.01 µL increments with the small loop capillary 0.01 – 40 µL in 0.01 µL increments with the extended loop capillary |
Precision | Typically < 0.5 % RSD of peak areas from 5 – 40 µL, Typically < 1 % RSD from 1 – 5 µL Typically < 3 % RSD from 0.2 – 1 µL Variable volume |
Sample viscosity range | 0.2 – 5 cp |
Sample capacity | 2 well plates (96 and 384) plus 10 additional 2-mL vials 108 x 2-mL vials in 2 x 54 vial plate plus 10 additional 2-mL vials 30 x 6-mL vials in 2 x 15 vial plate plus 10 additional 2-mL vials 54 Eppendorf tubes (0.5/1.5/2.0mL) in 2 x 27 Eppendorf tube plate Also compatible with the Agilent 1200 Series sample capacity extension for further expansion of the sample capacity |
Injection cycle time | Typically < 30 s using following standard conditions: Default draw speed: 4 µL/min Default eject speed: 10 µL/min Injection volume: 0.1 µL |
Carry-over | Typically < 0.05 % using the following conditions: Column: 150 x 0.5 mm Hypersil ODS, 3 µm Mobile phase: Water/Acetonitrile = 85/15 Column Flow rate: 13 µL/min Injection volume: 1 µL caffeine (=25ng caffeine), 1 µL water to test carryover Outside wash of needle before injection: 20 sec with water using flush port |