The Agilent 6230 time-of-flight LC/MS (LC/TOF) system allows you to upgrade from unit mass measurements to accurate mass for even more confident compound identification beyond conventional LC/MS detection. You will get full-spectrum data for screening, profiling and identification of small molecules, large biologic compounds, and everything in between, making the 6230B HPLC TOF ideal for applications like drug development, toxicology, and the analysis of intact proteins.
MS/MS applications are also supported by using fragments formed in the desolvation region of the TOF system ion source. All Ions MS/MS software then allows highly confident target compound identification by matching fragmentation patterns with an Agilent MS/MS library. Other advanced and intuitive software tools like one-click SWARM autotune and Walkup software allow anyone to routinely achieve reliable results.
Features
- Reduce false-positives with better than 1 ppm mass accuracy
- Ensure excellent data quality even for fast UHPLC peaks in high-throughput workflows with data acquisition rates up to 44 spectra/second
- Identify compounds based on accurate mass, isotopic abundance, and chemical composition using Find by Formula in MassHunter software
- Analyze monoclonal antibodies (mAb) with high mass accuracy and resolving power for glycoforms and truncation
- Characterize large biologic compounds with a broad mass range of up to 20,000 m/z
- Separate target compounds from interferences with high-resolution data
- Find impurities at extremely low concentrations, using Agilent Jet Stream technology with low picogram on-column sensitivity
- Identify trace-level target compounds in the presence of more abundant matrix compounds with up to five orders of in-spectrum dynamic range
- Reduce administrative tasks and risks while eliminating the need for LC/MS expertise with intuitive MassHunter Walkup software
- Perform untargeted screening with optional fragmentation data using in-source induced dissociation
- Go beyond UHPLC separation with support for capillary electrophoresis, SFC, 2D-LC, and even GC-APCI chromatographic interfaces
The Agilent 6230 Accurate-Mass TOF LC/MS achieves sensitivity, mass accuracy, mass resolution, and scan speed specifications after autotune without manual user intervention.
| Parameter | Measure | Specification |
| MS sensitivity with AJS | 1 pg reserpine injected on-column Measured at m/z 609.2807 | Signal-to-noise ratio > 50:1
Noise = 1 × RMS |
| MS sensitivity with ESI (Option 200) | 1 pg reserpine injected on-column Measured at m/z 609.2807 | Signal-to-noise ratio > 10:1
Noise = 1 × RMS |
| Mass resolving power | Measured at m/z 1,522 after automatic tuning procedure | 22,000 FWHM |
| Mass accuracy–MS mode | Measured at m/z 609.2807 using an internal reference mass | < 1 ppm |
| Spectral acquisition rate, MS mode | Spectra acquisition from m/z 100 to 3,200 | 30 spectra/second |
| Dynamic range | In-scan dynamic range on co-eluting components | Up to 5 decades |
| Mass range | m/z 25 to 20,000 | |
| Polarity switching | Pos/neg switching | < 1 sec |
| Mass accuracy temperature stability–MS mode | Temperature: 15 to 35 °C (59 to 95 °F) at constant temperature while setting autotune at 25 °C | Maintain 2 ppm mass accuracy within 2 °C drift per hour |
General system specifications
| Parameter | Measure |
| Single point of control | Single point data system method capability with full control for HPLC system and TOF and communication between the two such that if the LC or MS experiences a not ready condition, error, or leak, the system stops injecting the sample. |
| Wide range of ionization sources | • Electrospray (ESI)
• Atmospheric pressure chemical ionization (APCI) • Atmospheric pressure photoionization (APPI) • Multimode source (simultaneous ESI and APCI) • HPLC-Chip/MS interface • AP/MALDI PDF + source based upon pulsed dynamic focusing |
| Transparent introduction of reference masses | • Dual-sprayer orthogonal ESI source, with one sprayer for analytical flow and one for the reference compound
• Built-in calibrant delivery system for automated introduction of reference masses • Real-time internal reference mass correction for MS operation • Internal reference mass correction for heated sources–multimode, APCI, APPI |
| Autotune | Automated optimization of ion optics and full TOF MS mass axis calibration |
| Data mining tools | One-button extraction of compound-specific spectral and chromatographic information using Find Compounds algorithms |
| Linked software to enable quick manual review of complex data sets | Compound-based data browsing with dynamic links to spectra and chromatograms |
| Software for protein identification | Full compatibility with optional Spectrum Mill for MassHunter Workstation, including optimized Data Extractor |
| Software for verification of recombinant software proteins or synthetic peptides | BioConfirm |
| Software for walk up compound analysis | Easy Access software |
| Molecular profiling tools | • Measurement of potentially relevant molecular features from one or more LC/MS analyses (MassHunter)
• Differential expression analysis to reveal features that separate one sample set from another (optional MassHunter Mass Profiler) |
| Safety | • Canadian Standards Association
^CAN/CSA-C22-2 No. 61010-01-4 ^CSA/Nationally Recognized Test Laboratory (NRTL) UL 61010-1:2005 • International Electrotechnical Commission (IEC) 61010-1 • EuroNorm (EN): 61010-1 |
| Electromagnetic compatibility | CISPR11/EN55011: Group 1, Class A |
| Sound emission | EN 27779:1991 – sound pressure Lp < 70 db |
| Power | • Americas and Japan: 200 to 220 VAC; 2500 VA max, 50/60 Hz, 15 A circuit
• Europe and Asia Pacific: 220 to 240 VAC; 2500 VA max, 50 Hz, 15 A circuit |
| Operating environment | Humidity: 20 to 85 % |
| Size | 83 cm long × 73 cm wide × 133 cm high |
| Weight | 260 pounds |
