This system includes:
- (1) Agilent 1290 G4220A Binary Pump
- (1) Agilent 1290 G4226A Infinity Sampler
- (1) Agilent 1290 G1330B Autosampler Thermostat
- (1) Agilent 1290 G1316C Thermostatted Column Compartment (TCC)
- (1) Agilent 1290 G4212A Infinity Diode Array Detector (DAD)
Agilent 1290 G4220A Binary Pump
| Type | Specifications |
|---|---|
| Hydraulic system | Two dual pistons in series pumps with proprietary servo-controlled variable stroke design, power transmission by ball screws, smooth motion control of pistons for active damping. |
| Settable flow range | Setpoints 0.001—5 ml/min, in 0.001 ml/min increments. Executed in 300 pl/step increments |
| Flow precision | ≤0.07 % RSD or 0.005 min SD, whatever is greater (0.2—5.0 ml/min). Based on retention time at constant room temperature. |
| Flow accuracy | ±1 % or 10 μl/min, whatever is greater. Pumping degassed H2O at 10 MPa (100 bar) |
| Pressure operating range | Operating range up to 120 MPa (1200bar), up to 2ml/min, 80MPa (800 bar) at 5 ml/min. |
| Pressure pulsation | <1 % amplitude or < 0.5 MPa (5 bar), whatever is greater. At 1 ml/min water |
| Compressibility compensation | Automatic, pre-defined, based on mobile phase selection. |
| Gradient formation | High pressure binary mixing. |
| Delay volume | Jet Weaver V35: <45 μl Jet Weaver V100: <75 μl |
| Composition range | Settable range: 0 – 100 % |
| Composition precision | <0.15 % RSD, or 0.01 min SD, whatever is greater. 0.2 —5.0 ml/min; based on retention time at constant room temperature |
| Composition accuracy | ±0.35 % absolute (5 – 95 %, 0.2 – 5.0 ml/min) |
| Solvent selection valve | Default |
| Integrated degassing unit | Number of channels: 2Internal volume per channel: 1.5 mL |
| Control | Agilent ChemStation for LC (B.04.02 or above) EZChrom Elite (3.3.2 SP1 or above) OpenLAB (3.3.2 SP3) Masshunter (B.02.01 SP1 or above) |
| Local control | Agilent Instant Pilot (G4208A) (B.02.08 or above) |
| Communications | Controller-area network (CAN), RS232C, APG remote: ready, start, stop and shutdown signals, LAN |
| Safety and maintenance | Extensive diagnostics, error detection and display (through Agilent Lab Advisor), leak detection, safe leak handling, leak output signal for shutdown of the pumping system. Low voltage in major maintenance areas. |
| GLP features | Early maintenance feedback (EMF) for continuos tracking of instrument usage in terms of purge valve switches and volume of pumped mobile phase with pre-defined and user settable limits and feedback messages. Electronic records of maintenance and errors. |
| Housing | All materials recyclable. |
Agilent 1290 G4226A Infinity Sampler
| Precision | Typically <0.25 % RSD from 5 – 20 µL Typically <0.5 % RSD from 2 – 5 µL volume Typically <0.7 % RSD from 1 – 2 µL volume |
| Pressure range | Up to 1200 bar |
| Injection range | 0.1 – 20 µL in 0.1 µL increments |
| Sample viscosity range | 0.2 – 5 cp |
| Sample capacity | 2 x well plates (MTP) + 10 x 2 mL vials |
| Carry over | Typically <0.004 % |
| Injection cycle time | Typically <21 s using following standard conditions:Default draw speed: 100 µL/min Default eject speed: 100 µL/min Injection volume: 5 µL |
Agilent 1290 G1330B Thermostat
| Temperature range | Settable from 4°C to 40°C in 1°C increments |
| Temperature accuracy at ambient temperatures < 25°C and humidity < 50% |
3°C to 8°C at a setpoint of 4 °C |
| Temperature accuracy at ambient temperatures <30°C and humidity <60% |
3°C to 9°C at a setpoint of 4°C |
Agilent 1290 G1316C Thermostatted Column Compartment
- Temperature precision specification of ± 0.05 degrees for repeatable retention times and peak areas.
- Maximum application flexibility through Peltier cooling and heating for two independent temperature zones from 10°C below ambient temperature up to 100°C.
- Low dispersion – optimized for low internal volume contribution.
- Spacious column racks provide room for three 30 cm columns or several shorter columns.
- Ultra high pressure valves for many applications such as alternating column regeneration, sample preparation and dual or multi-column selection.
- Unique pull-out and Quick Change Valve design for simplistic plumbing and valve exchange.
- Clustering of up to three 1290 Infinity TCC modules for maximum flexibility for multi-method applications such as automated method development.
- Further features include a door-open sensor, improved thermal insulation, leak funnel and capillary guide.
Agilent 1290 G4212A Infinity Diode Array Detector (DAD)
- Ultra sensitivity through revolutionary Agilent Max-Light cartridge cell with 60 mm
- optical path length (typically noise: < ± 0.6 μAU/cm).
- Universal Agilent Max-Light cartridge standard cell with 10 mm optical path length provides high sensitivity (noise: < ± 3 μAU) and lowest peak dispersion for 2.1, 3 and 4.6 mm ID columns.
- Programmable slit from 1 to 8 nm provides optimum incident light conditions for rapid optimization of sensitivity, linearity and spectral resolution.
- Multiple wavelength and full spectral detection at high sampling rate of 160 Hz, keeping pace with fastest possible analysis speed.
- More reliable and robust peak integration process due through less baseline drift.
- Full spectral detection for compound identification by spectral libraries or verification of the separation quality with peak purity analysis for ultra fast LC. Simultaneous detection of up to 8 signals for increased sensitivity and selectivity.
- Wide linear range (typically up to 2.5 AU) – for reliable, simultaneous quantification of primary compounds, by-products and impurities.
- Radio frequency identification (RFID) technology for flow cells and lamp provide new levels of data security and traceability.
- Electronic temperature control (ETC) provides maximum baseline stability and practical sensitivity under fluctuating ambient temperature and humidity conditions.
- Reference wavelength for elimination of background interference.
