The 1260 Infinity II Bio-inert Fraction Collector is the ideal extension to your system when you need to investigate separated compounds further. It fits perfectly in Agilent‘s modular LC design and does not require extra bench space. Patented fraction delay calibration and time- or peak-triggered fraction collection facilitates superior recovery and purity.
A wide range of collection vessels, including vials, microtiter plates or custom vessels, provide highest collection flexibility. With flow rates up to 10 mL/min even run separations using columns with larger inside diameters for small scale prep applications are possible. An additional cooling module is available to prevent degradation of thermally-labile bio-molecules.
Specifications:
| Type | Specifications |
|---|---|
| Delay volume | Fraction collector inlet to diverter valve: ~50 (typical, depends on length of the tubing) Diverter valve: ~15 Diverter valve to needle: ~10 Needle: ~4 |
| Minimum system flow | Depending on the recommended flowrates of the installed tubing kit |
| Maximum system flow | 10 mL/min |
| Maximum collection volume | ~20 mL with 30 x 48 mm (OD x L) tube |
| Maximum capacity | 3 fraction collectors in parallel plus one recovery fraction collector |
| Cooling | Optional (with additional G1330B), performance depending on ambient conditions and the volume of collected fractions |
| Trigger modes | Time slices Peak (threshold, up- / downslope) Timetable (combination of time intervals and peak) Manual trigger (supported only with Agilent Instant Pilot G4208A) |
| Operating Modes | Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using deep well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste Needle into location: Needle pushes into the vessel as deep as specified, for the use with capped vials and test tubes and well plates with closing mats Droplet setup mode: enables the fraction collector to collect small fractions without bubbles. The tip of the fraction collector needle initially moves down to the bottom of the well. Then it slowly moves upwards while the fraction is collected. |
| Maximum time to move between neighboring vessels | Movement in x-direction: < 0.15 s Movement in y-direction: < 0.3 s |
| Diverter valve | 3/2 valve, with switching time < 100 ms |
| Maximum pressure | 6 bar at the diverter valve during switching |
| Plates/Trays | 4 x well-plates full tray (MTP) 2 × well-plates std. tray + 10 funnels with external containers (+ 1 half tray) 2 × well-plates std. tray (MTP) + 10 × 2 mL vials (+ 1 half tray) 100 x 2 mL in std. tray (+ 1 half tray) 3 x 40 x 2 mL in half tray 3 x 40 funnels in half tray 3 x 15 x 6 mL in half tray Full tray with 40 test tubes (30 mm OD, max. height 48 mm, ~20 mL / tube) Full tray with 60 test tubes (25 mm OD, max. height 48 mm, ~15 mL / tube) Full tray with 126 test tubes (16 mm OD, max. height 48 mm, ~11 mL / tube) Full tray with 215 test tubes (12 mm OD, max. height 48 mm, ~8 mL / tube) Installed trays are automatically detected and identified. In operation mode “Needle into location” installed plates and vials can be detected. Only one type of well-plates can be used at a time in one tray |
| Fraction Containers | 30 x 48 mm (OD x L) tubes, ~20 mL / tube 25 x 48 mm (OD x L) tubes, ~15 mL / tube 16 x 48 mm (OD x L) tubes, ~11 mL / tube 12 x 48 mm (OD x L) tubes, ~8 mL / tube Vials, well plates, capped vials, and well plates with closing mats can be used as recommended by Agilent Technologies |
| Maximum tube height | 48 mm (with bio-inert needle assembly G5667-87200) |
| Instrument Control | LC and CE Drivers A.02.17 or above Instrument Control Framework (ICF) A.02.04 or above Instant Pilot (G4208A) with firmware B.02.22 or above Lab Advisor B.02.10 or above |
| Communications | Controller-area network (CAN), Local Area Network (LAN) ERI: ready, start, stop and shut-down signals |
| Maintenance and safety-related features | Extensive diagnostics, error detection and display with Agilent Lab Advisor software Leak detection, safe leak handling, leak output signal for shutdown of pumping system, and low voltages in major maintenance areas |
| GLP features | Early maintenance feedback (EMF) for continuous tracking of instrument usage with user- settable limits and feedback messages. Electronic records of maintenance and errors |
| Housing | All materials recyclable |
