Type

Specification

Detection type

Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis

Performance

Specifications

10 fg Anthracene,

Ex=250 nm, Em=400 nm*

RAMAN single wavelength

(H2O) > 500 with Ex=350 nm,

Em=397 nm, dark value 450 nm,

standard flow cell

time constant=4 seconds

(8 seconds responsetime)

RAMAN dual wavelength (H2O)

> 300 with Ex=350 nm,

Em=397 nm, dark value 450 nm,

standard flow cell

time constant=4 seconds

(8 seconds responsetime)

Light source

Xenon Flash Lamp, normal mode 20 W,

economy mode 5 W

Pulse frequency

296 Hz for single signal mode

74 Hz for spectral mode

Excitation

Monochromator

Range:200 nm – 700 nm and zero-order

Bandwidth:20 nm (fixed)

Monochromator:concave holographic grating, F/1.6, blaze: 300 nm

Emission

Monochromator

Range:280 nm – 900 nm and zero-order

Bandwidth:20 nm (fixed)

Monochromator:concave holographic grating, F/1.6, blaze: 400 nm

Reference System:

in-line excitation measurement

Timetable programing

up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectralparameters

Spectrum acquisition:

Excitation or Emission spectra

Scan speed: 28 ms per datapoint

(e.g. 0.6 s/spectrum 200-400 nm, 10 nm step)

Step size: 1-20 nm

Spectra storage: All

Wavelength

Characteristic

Repeatability+/- 0.2 nm

Accuracy+/- 3 nm setting

Flow cells

Standard: 8 µl volume and 20 bar

(2 MPa) pressure maximum, quartz

Optional: Fluorescence cuvette for offline

spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz

Control and data

Evaluation

Agilent ChemStation for LC, Agilent Instant Pilot G4208A or Agilent Control Module G1323B with limited spectral data analysis and printing of spectra

Analog outputs

Recorder/integrator: 100 mV or

1 V, output range >102

 luminescence units, two outputs

Communications

Controller-area network (CAN),

GPIB, RS-232C, LAN, APG

Remote: ready, start, stop and

shut-down signals

Safety and

Maintenance

Extensive diagnostics, error detection and display (through Instant Pilot G4208A, Control Module G1323B and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas

GLP features

Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages.

Electronic records of maintenance and errors. Verification of wavelength accuracy, using the Raman band of water.

Housing

All materials recyclable.

Environment:

0 to 40 °C constant temperature at <95% humidity (non-condensing)

Dimensions:

140 mm x 345 mm x 435 mm

(5.5 x 13.5 x 17 inches)

(height x width x depth)

Weight:

11.5 kg (25.5 lbs)