| Solvent Management | |
| Number of Solvents | 1 to 4 |
| Solvent conditioning | Vacuum degas, two operating modes, four chambers, < 500µl internal volume per chamber 0.000 to 10.000 mL/min (0.050 to 5.00 mL/min typical) in 0.001 mL/min increments |
| Compressibility compensation | Automatic and continuous |
| System delay volume | < 650 µL, independent of backpressure at 1mL/min |
| Plunger seal wash | Integral, active, programmable |
| Gradient profiles | 11 gradient curves including linear, step (2), concave (4), and convex (4) |
| Dry prime/wet prime | Automatic front panel control, SystemPrep function for automatic solvent(s) purge |
| Flow ramping | Time (0.01 to 30.00 min in 0.01 min increments) to reach maximum flow rates |
| Maximum operating pressure | 5000 psi (345 bar) (0.010 to 3.000 mL/min) programmable upper and lower limits |
| Composition range | 0.0% to 100.0%, in 0.1% increments |
| Composition accuracy | ±0.5% absolute, independent of backpressure (proportioning valve pair test, [degassed methanol: methanol/propylparaben, 2.0mL/min, 254nm]) |
| Composition precision | ≤0.15% RSD or ≤0.02 min SD, whichever is greater, based on retention time (degassed methanol: water 60:40 dial-a-mix, 1.00mL/min, six replicates, phenone mix, 254nm) |
| Flow precision | ≤0.075% RSD or ≤0.02 min SD, six replicates, based on retention time or volumetric measures (0.200 to 5.000 mL/min), isocratic premix |
| Flow rate range | 0.000 to 10.000 mL/min (0.050 to 5.000 mL/min typical) in 0.001mL/min increments |
| Flow accuracy | ±1% or 10µl/min whichever is greater, 0.200 to 5.000 mL/min (degassed methanol, at 600 psi backpressure) |
| Sample Management | |
| Number of sample vials | 120 vials, configured in five carousels of 24 vials each |
| Number of sample injections | 1 to 99 injections per sample vial |
| Sample delivery precision | Typically <0.5% RSD, 5 to 80µl (degassed methanol: water 60:40 dial-a-mix, 1mL/min, six replicates, phenone mix, 254 nm) |
| Sample carryover | ≤ 0.01% for caffeine, under specified conditions |
| Injection needle wash | Integral, active, programmable |
| Injection accuracy | ± 1µL (±2%) (50µL, N=6), sample: 100% degassed water, analytical solvent: 100% degassed methanol |
| Standard sample vial | 2mL |
| Advanced operations | Priority samples, auto additions, auto standards |
| Injection volume range | 0.1 to 100.0µL, standard; 0.1 to 2000.0µL, with optional sample loop |
| Injection linearity | > 0.999 coefficient of deviation (1.000 to 100.000µL) |
| Minimum sample required | 10µL, using low volume inserts |
| Sample temperature control (optional) | 4 to 40°C, programmable in 1°C increments |
| Column heater(optional) | 20 to 65°C, in 1°C increments (5°C above ambient) |
| Column heater/cooler (optional) | Ambient minus 15°C or 4°C (whichever is greatest) up to 65°C, in 1°C increments |
This refurbished Waters Alliance 2695 HPLC system with Waters 2420 ELS Detector, or Waters 2996 PDA Detector, or Waters 2414 RID, or Waters 2475 Multi-Wavelength Fluorescence Detector, or Waters 2489 UV/Vis Detector, or Waters 2465 Electrochemical Detector are all in great condition. it have been tested at SpectraLab.
A 90 day warranty is included.
