This system includes:
- (1) Thermo Scientific Velos Pro
- (1) Thermo Scientific Orbitrap Elite
- (1) Thermo Scentific Neslab ThermoFlex 900
- (1) Thermo Scientific ETD
- (2) Rough pump
- (1) PC with software
- (Optional) HPLC front end
Thermo Scientific Velos Pro
- Resolving power up to 100,000 FWHM and mass accuracy better than 1ppm RMS provide confidence when analyzing complex samples
- Multiple fragmentation techniques—CID, HCD, and optionally ETD—offer versatility and facilitate structural characterization
- Fast scanning and parallel MS and MSn analysis speeds analyses
- Ion optics with novel neutral-blocking technology increase robustness
- Collision cell with axial field gradient improves quantitative results
- High mass resolution and mass accuracy enable high-confidence molecular weight determination of intact proteins, peptide identification in complex samples and analysis of isobaric species
- High sensitivity increases detection of low-abundance peptides and proteins
- Short duty cycle increases sequence coverage within in a single experiment
- Stability, mass accuracy and wide dynamic facilitate label-free quantification
- Mass resolution and mass accuracy distinguish between heavy and light peptide ion pairs and interferences in SILAC experiments
- HCD cell with axial field improves quantification of isobarically labeled peptides
- Optional electron-transfer dissociation (ETD) generates complementary information for P™ analyses and de novo sequencing
Thermo Scientific Orbitrap Elite
Analyze demanding proteomics, metabolomics, lipidomics and metabolism samples in greater depth using the Thermo Scientific™ Orbitrap Elite™ Hybrid Ion Trap-Orbitrap Mass Spectrometer. This instrument combines a high-field Thermo Scientific™ Orbitrap™ mass analyzer and Thermo Scientific™ Velos Pro™ ion trap technology to deliver very high resolution, speed, sensitivity and complementary fragmentation information.
Mass Range | 50 to 2000m/z; 200 to 4000m/z |
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Scan Rates | MSn for n = 1 Through 10 |
Weight (Metric) | 620kg |
Dynamic Range | >5000 within a single scan guaranteeing specified |
Mass Accuracy | <3ppm RMS with external calibration; <1ppm RMS using internal calibration; <1ppm RMS using internal calibration |
Resolution | 60,000 at m/z 400 at a scan rate of 4Hz, min. resolution 15,000, max. resolution >240,000 at m/z 400 |
Voltage | 230V |
Item Description | Orbitrap Elite hybrid ion trap-Orbitrap mass spectrometer |
Description | Orbitrap Elite Hybrid Ion Trap-Orbitrap Mass Spectrometer |
Unit Size | Each |
Thermo Scentific Neslab ThermoFlex 900
Optimized for the most demanding applications, Thermo Scientific™ ThermoFlex Recirculating Chillers are reliable, easy-to-use and easy-to-maintain. The ThermoFlex platform was developed with customer input from concept to design. This high-performance chiller platform, with cooling capacity up to 24,000 watts (81,948 btu/hr), is configurable to meet application demands.
Configuration Options | Fluid Filtration (included), DI Support (Available), Auto Refill (Available), Flow Control with Readout (Available) |
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Description | ThermoFlex 900 Recirculating Chiller |
Temperature Stability | &plusnm;0.1°C |
Certifications/Compliance | CE, CSA |
Communications | Analog & Digital (available) |
Condenser Type | Air Cooled |
Cooling Capacity | Up to 900 watts (60Hz) 750 watts (50Hz) |
Pump Capacity | Up to 3.3 gpm @ 60 psig (12.5 L/min. @ 4.1 bar) (50Hz) Up to 4.0 gpm @ 60 psig (15. |
Temperature Range | 5°C to 40°C |
Unit Size | Each |
Thermo Scientific ETD
Achieve better analysis of proteins and their post-translational modifications (PTMs) with the Thermo Scientific™ Electron Transfer Dissociation (ETD) option. Unlike CID, which cleaves weakly bound modifications off the peptide backbone, ETD induces fragmentation along the peptide backbone in a sequence-independent manner, leaving labile modifications linked to the peptide chain. This greatly simplifies identification of modification sites. ETD produces primarily c- and z-type fragment ions that complement the b- and y-type ions produced by CID, increasing sequence coverage and protein IDs.
- ETD preserves labile PTMs, allowing straightforward identification of the peptide sequence and the site of modification
- For top-down analysis, ETD can be combined with proton transfer reaction (PTR) which dramatically simplifies spectra from intact proteins
- For workflows that include low-mass isobaric mass tags, ETD can be combined with pulsed-Q dissociation (PQD) which eliminates the normal low-mass cutoff
- ETD can be added to most Thermo Scientific LTQ-series ion trap and hybrid ion trap-Orbitrap mass spectrometers
- Proteome Discoverer software includes the proprietary Z-Core database search algorithm specifically optimized for ETD spectra
- Proteome Discoverer software can easily merge complementary CID and ETD results to maximize protein sequence coverage