Wyatt Technologies microDAWN Multi-Angle static Light Scattering (MALS) Detector [PN: WMD03-01]
The microDAWN MALS detector performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects the microDAWN is similar to the well-established miniDAWN, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius.
The microDAWN is uniquely suitable for use in conjunction with UHPLC Size Exclusion Chromatography (SEC-MALS) thanks to its small interdetector dispersion. In some cases it may be used with ion-exchange or other types of chromatography. The microDAWN produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC.
| Measurements | |
| Molar Mass Range | 200 Da to 10 MDa (proteins) or 1 MDa (linear polymers)1 |
| Molecular or Particle Size Range (Radius, Rg) | 10 nm to 50 nm, up to 150 nm with shape-specific models |
| Molecular Size Range (Rh) | In flow mode, 0.5 nm to 30 nm; batch mode 0.5 nm to 1 μm (requires WyattQELS DLS module or DynaPro NanoStar + fiber optic connection)2 |
| Sensitivity | 3.5 ng of 100 kDa polystyrene in THF or 70 ng of BSA in PBS, assuming a 15 cm UHPLC-SEC column and no post-column filter |
| Electronics | |
| Analog Inputs | 2 differential analog inputs with 24 bit resolution; Input range -10 V to +10 V |
| Analog Outputs | 1 analog output from user selectable measurements channels -10 V to +10 V |
| Other Inputs/Outputs | Alarm in, Alarm out/retransmit, Auto-inject in, Auto inject contact closure retransmit, Recycle In/Out (operates Orbit recycle valve) |
| Dimensions | 58 cm (L) x 36 cm (W) x 18 cm (H) |
