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Triple Quadrupole Operation, Troubleshooting & Maintenance

Triple Quadrupole Operation, Troubleshooting & Maintenance

This is an excellent hands-on training course in LC-MS/MS, a rapidly growing technique, designed to introduce instrument operation, troubleshooting and maintenance of an LC/MS System.  Modern equipment is used in conjunction with practical aspects providing strong grounding in LC-MS/MS instrumental techniques. A brief outline of the program is as detailed below:

·        Overview of LC-MS, Single and Triple-Quad instrumental techniques

·        HPLC Considerations for LC-MS/MS

·        Flow Rate and Flow Splitting Considerations

·        Electrospray Ionisation (ESI) Theory & Instrumentation

·        Atmospheric Pressure Ionisation (API) Theory & Instrumentation

·        Calibrating and Tuning

·        Tuning and Optimising the ESI Interface Parameters

·        Choosing API Mode, Alternative Sources and Multi-mode Sources

·        Designing Spectral Experiments – Scan, SIM and MRM Acquisition for Qualitative and Quantitative Operation

·        Quantitative Analysis using the MS Detector (Calibration, LOD, etc.)

·        Qualitative Analysis using the MS Detector (In-house Libraries/Peak ID and Library Searching)

·        In-Source Collision Induced Dissociation (CID)

·        Introduction to Spectral Interpretation

·        MS Diagnostics and Introduction to LC-MS Routine Maintenance


Course Type: Hands-On

Level: Intermediate

Duration: 2 days


Option 1: PE Sciex API 4000/3000/2000/365 or Sciex 2000 Qtrap LC/MS/MS System*

Training Code: TC-MS-0004/001

Option 2: Thermo Finnigan LCQ Classic/Duo/Deca LC/MS/MS System*

Training Code: TC-MS-0004/002

Option 3: Thermo Finnigan TSQ 7000 or TSQ Quantum LC/MS/MS System

Training Code: TC-MS-0004/003

Option 4: Micromass Quattro LC/MS/MS System

Training Code: TC-MS-0004/004

Option 5: Varian 1200L Quadrupole LC/MS/MS System

Training Code: TC-MS-0004/005

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