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Agilent 1200 Series Prep HPLC

  HP-1SY-0750
 
Agilent 1200 Series Prep HPLC
  Features:
  • Dedicated systems with low delay volumes optimized for high recovery and purity and desired throughput
  • Software solutions for different user needs allows you to configure your own purification system
  • Fraction collection based on time, peak, and/or masses (or even manually) allows you to collect only the desired fractions
  • Automated delay volume calibration for easy and fast system set-up
This Agilent 1200 PrepHPLC includes:
Agilent 1200 Series Preparative Pump G1361A

Type

Specification

Hydraulic system

Dual pistons in parallel

Flow range Settable

0.001 – 100 ml/min

Flow precision

<0.5% RSD

Pressure range

20 to 400 bar (5880 psi) system pressure

Compressibility compensation

User-selectable, based on mobile phase compressibility

Recommended pH range

1.0 – 12.5, solvents with pH <2.3 should not contain acids which attack stainless steel

Control and data evaluation

Agilent ChemStation for LC and LC/MS

Communication

Controller-area network (CAN), RS-232, APG Remote: ready, start, stop and shut down signals, CAN-DC OUT, LAN optional

Safety and maintenance

 

Extensive diagnostics, error detection and display (through control

module and Agilent ChemStation), leak detection, safe leak handling,

leak output signal for shutdown of pumping system. Low voltages in

major maintenance areas.

GLP features

Early maintenance feedback (EMF) for continuous tracking of

instrument usage in terms of seal wear and volume of pumped mobile

phase with user-settable limits and feedback messages. Electronic

records of maintenance and errors.

Housing

All materials recyclable


Agilent 1200 series Fraction Collectors G1364B

Type

Specification

trigger modes

Time slices,

Peak (threshold, up- / downslope),

Timetable (combination of time intervals and peak) and

Manual trigger (supported only with G1323B Control Module)

Agilent 1200 Series DAD/MWD detectors (G1315A/B/C, G1365 A/B/C),

the Agilent 1200 Series fluorescence detector and the Agilent G1946C/D,

G1956A/B LC-MSD are fully supported other detectors can be used but are

not supported for fraction collection.

operating modes

Discrete fractions: default mode for all vessels. The flow is diverted to

waste, while moving from one vessel position to the next vessel position

Continuous flow: optional, available only when using well plates. It is

possible to move from one well plate position to the next one without

diverting the flow into the well plate to waste

Fraction capacities and trays

4 x well-plates full tray (MTP)*

 (for use with deep well plates, only)

2 × well-plates std. tray (MTP) (for use with deep well plates, only)

+ 10 × 2 ml vials* (+ 1 half tray)

100 x 2 ml in std. tray (+ 1 half tray)*

3 x 40 x 2 ml in half tray*

3 x 15 x 6 ml in half tray*

Full tray with 40 test tubes (30 mm OD, max. height 100 mm, ~45 ml / tube)

Full tray with 60 test tubes (25 mm OD, max. height 100 mm, ~25 ml / tube)

Full tray with 126 test tubes (16 mm OD, max. height 100 mm, ~12 ml /

tube)

Full tray with 215 test tubes (12 mm OD, max. height 100 mm, ~7 ml / tube)

Installed trays are automatically detected and identified.

For the with uncapped vials, tests tubes and well plates, only!

test tube / plate sizes

Minimum 48 mm to 100 mm maximum

Maximum tube Volume

ca. 45 ml

Maximum flow rate

100 ml/min (depending on viscosity and generated back pressure, max, 6 bar at the diverter valve)

Delay volumes [µl]

Fraction collector inlet to diverter valve: ~500 (typical, depends on length of

the tubing)

Diverter valve: ~15

Diverter valve to needle: ~110

Needle: ~5

Delay calibration sensor

Single wavelength absorbance detector working at 654 nm, consisting of a

LED and a photo diode

Diverter valve

3/2 Diverter valve with low internal volume (15 μl), switching time

< 100 ms, maximum operating pressure 6 bar

cooling

Optional (with additional G1330B), performance depending on ambient

conditions and the volume of collected fractions

maximum capacity

3 fraction collectors in parallel plus one recovery fraction collector

connected via 12-Position, 13-Port Selector valve (PN G1160A

GLP features

Early maintenance feedback (EMF), electronic records of maintenance and

errors

Interfaces

Controller-area network (CAN).

optional; LAN or external contacts interface

RS232C,

APG-remote (for remote start / stop signals to / from other modules)

Interface to G1330A Thermostat

CAN-DC-out for operation of Agilent

approved external devices like valves

Safety features

Leak detection and safe leak handling, error detection and display, exhaust

fan for fume extraction of hazardous vapors


Agilent 1200 Series DualLoop Autosampler G2258A

Type

Specification

GLP features

Early maintenance feedback (EMF), electronic records of maintenance and

errors

Communications

Controller-area network (CAN). RS232C, APG-remote standard,

optional: four external contact closures and BCD vial number output

optional: LAN communication card

Instrument control

Agilent ChemStation software

Agilent HT Purification SW

Agilent G1323B Control Module

Safety features

Leak detection and safe leak handling, low voltages in maintenance areas,

error detection and display

Injection modes

Partial loop filling

Complete loop filling

Injection range

0.1 – 5000 µl in 0.1 µl increments

Recommended injection range 10 - 5000 µl

Area precision Complete Loop Fill

Typically < 0.5% RSD,(50 µl loop, overfill factor 3 or greater)

Area precision Partial Loop Fill

Typically < 1.0% RSD,(5000 µl loop, 1000µl injection volume, draw/eject

speed 20 ml/min)

Carry-over

< 0.1% with rinse factor > 3 (see test method listing)

Area precision Complete Loop Fill

Typically < 0.5% RSD,(50 µl loop, overfill factor 3 or greater)

Area precision Partial Loop Fill

Typically < 1.0% RSD,(5000 µl loop, 1000 µl injection volume, draw/eject

speed 20 ml/min)

Draw/eject speed

max. 50 ml/min (selectable in 1 µl/min steps)

Loop size

max. loop size 5 ml (selectable range: 5 µl - 50000 µl)

Sample viscosity range

0.2 – 5 cp

Injection volume

Selectable range: 0 - 50000 µl in 0.1 µl steps

Recommended range: 10 µl - 5000 µl in 1 µl steps

Temperature controlled version

with G1330B

Temperature range of sample compartment: 4 - 40 deg C

Sample capacity

2 × well-plates (MTP) + additional 10 × 2 ml vials

Supported sample

Plates

Any combination of 2 of the following plates:

96 well plate (shallow/deep/conical)

54 x 2 ml vial plate

15 x 6 ml vial plate

27 x Eppendorf plate for Eppendorf tubes of 0.5 ml,1.5 ml or 2.0ml

Maximum plate height with plate tray G2258-60001: 50 mm


Agilent 1200 SeriesPreparative Autosampler G2260A

Type

Specification

Pressure

Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5800psi)

GLP features

Early maintenance feedback (EMF), electronic records of

maintenance and errors

Communications

Controller-area network (CAN). GPIB (IEEE-448), RS232C,

APG-remote standard, optional four external contact closures and

BCD vial number output

Safety features

Leak detection and safe leak handling, low voltages in maintenance

areas, error detection and display

Injection range

0.1 – 900 µl in 0.1 µl increments (recommended 1 µl increments)

Up to 1800 µl with multiple draw (hardware modification required)

Up to 5000 µl with multiple draw (hardware modification required)

Replicate injections

1 – 99 from one vial

Precision

Typically < 0.5% RSD of peak areas from 5 – 2000 µl, Typically

< 1% RSD of peak areas from 2000 – 5000 µl, Typically < 3% RSD of

peak areas from 1 – 5 µl

Minimum sample volume

1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in

300 µl microvial

Sample viscosity range

0.2 – 50 cp

Sample capacity

100 × 2-ml vials in 1 tray

15 × 6-ml vials in ½ tray (Agilent vials only)

Injection cycle time

Typically 50 s, depending on draw speed and injection volume


Agilent 1200 SeriesG1315B DAD

Wavelength range

190-950 nm

Light source

Deuterium and tungsten lamps

Detection type

1024 - element photodiode array

Short term noise (ASTM) single & mult-wavelength

± 1° x 10-5 AU at 254 and 750 nm

Drift

2° x 10-3 AU / hr at 254 nm

Linear absorbance range

> 2 AU (upper limit)

Wavelength accuracy

± 1 nm

Wavelength bunching

1 - 400 nm

Diode width

< 1 nm

Flow cells

Standard: 13µl volume 10 mm cell path length and 120 bar (1760 psi) pressure maximum
Semi-Micro: 5µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
Micro: 2µl volume, 3 mm cell path length and 120 bar (1760 psi) pressure maximum
High Pressure: 1.7µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure max.
80 nano: 0.08µl volume, 100 mm cell path length and 50 bar (725 psi) pressure max.
500 nano: 0.5µl volume, 10 mm cell path length and 50 bar (725 psi) pressure max.

Analog outputs

Recorder / integrator: 10 mV or 1 V, output range 
0.001 - 2 AU, two outputs

Control and data evaluation

Agilent Chemstation for LC

Slit width

1, 2, 4, 8, 16 nm

Communication types

CAN, GBIP, RS-232C, APG, Remote: ready, start, stop and shut down

Wavelength range

190-950 nm

Light source

Deuterium and tungsten lamps

Detection type

1024 - element photodiode array

Short term noise (ASTM) single & mult-wavelength

± 1° x 10-5 AU at 254 and 750 nm

Drift

2° x 10-3 AU / hr at 254 nm

Linear absorbance range

> 2 AU (upper limit)




This Agilent 1200 Prep HPLC is in great condition. It has been tested at SpectraLabScientific.

A 90-day warranty is included.

We have special expertise on analytical equipment. We encourage you to contactus to speak with one of our expert and professional team members.

Thanks for looking and we look forward to hearing from you!
 
 
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