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Agilent 1200 Series HPLC system (with Diode Array Detector & Degasser)

 
Agilent 1200 Series HPLC system (with Diode Array Detector & Degasser)
Agilent 1200 Series HPLC system (with Diode Array Detector & Degasser)
  This HP / Agilent 1200 Series HPLC system comes with the followings:
Agilent 1200 series G1379B Degasser

Type

Specification

Maximum flow rate

0 - 5 ml/min per channel

Number of channels

4

Internal volume per channel

Typically 1 ml per channel

Materials in contact with solvent

PTFE, FEP,PEEK

pH range

1 – 14

RS-232 output

For diagnostic purposes


Agilent 1200 Series G1312A Bin pump

Type

Specification

Hydraulic system

Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve

Setable flow range

Setpoints 0.001 – 5 ml/min, in 0.001 ml/min increments

Flow range

0.1 – 5.0 ml/min

Flow precision

<0.07% RSD, or <0.02 min SD whatever is greater, based on retention time at constant room temperature

Flow accuracy

+1% of 10µl/min whatever is greater

Pressure

Operating range 0 400 bar (0-5880 psi) up to 5 ml/min

Pressure pulsation

< 2% amplitude (typically < 1%), at 1 ml/min isopropanol, at all pressures > 1 MPa

Compressibility compensation

User-selectable, based on mobile phase compressibility

Recommended pH range

1.0 – 12.5, solvents with pH <2.3 should not contain acids which attack stainless steel

Gradient formation

High-pressure binary mixing, delay volume 180-480 µl without mixer, 600 – 900 µl with mixer, dependent on back pressure

Composition range

1 – 99 % or 5 µl/min per channel, whatever is greater

Composition precision

<0.5% absolute

Composition accuracy

0.15% RSD, at 1 ml/min

Control and data evaluation

Agilent ChemStation for LC

Analog output

For pressure monitoring, 2mV/bar, one output

Communications

Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional

Safety and maintenance

Extensive diagnostics, error detection and display (through handheld controllers G4208A, G1323B and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltage in major maintenance areas

GLP features

Early maintenance feedback (EMF) for continuous tracking of instruments usage in terms of seal wear and volume of pumped mobile phase with user-settable limits and feedback messages. Electronic records of maintenance and errors.

Housing

All materials recycleable



Agilent 1200 G1367B HiP ALS Autosampler

Type

Specification

GLP features

Early maintenance feedback (EMF), electronic records of maintenance and errors

Communications

Controller-area network (CAN). RS232C, APG-remote standard, optional four external contact closures and BCD vial number output

Safety features

Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display

Injection range

0.1 – 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification required)

Precision

Typically < 0.25% RSD from 5 – 100 µl, Typically < 1% RSD from 1 – 5 µl variable volume

Pressure range

400 bar (5880 psi)

Sample viscosity range

0.2 – 5 cp

Sample capacity

2 × well plates (MTP) + 10 × 2 ml vials 108 x 2-mL vials in2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6-mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials54 Eppendorf tubes (0.5/1.5/2.0mL) in 2 x 27 Eppendorf tube plate

Also compatible with the Agilent 1200 Series sample capacity extension for further expansion of the sample capacity

Injection cycle time

Typically < 30 s using following standard conditions: Default draw speed: 200 µl/min Default eject speed: 200 µl/min Injection volume: 5 µl

Carry-over

Typically < 0.01% using the following conditions: Column: 125 x 4 mm Hypersil ODS, 5 µm Mobile phase: Water/Acetonitrile = 80/20 Flow rate: 1 ml/min Injection volume: 1 µl caffeine (1 mg/ml), 5 µl water to test carryover Outside wash of needle before injection: 20 sec with water using flush port



Agilent 1200 series G1316A TCC


Type

Specification

Temperature range

10 degrees below ambient to 80 °C

up to 80 °C: flow rates up to 5 ml/min

Temperature stability

± 0.15 °C

Temperature accuracy

± 0.8 °C

± 0.5 °C (with calibration)

Column capacity

Three 30 cm

Warm-up/cool-down time

5 minutes from ambient to 40 °C

10 minutes from 40 – 20 °C

Dead volume

 

3 µl left heat exchanger

6 µl right heat exchanger

Communications

Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN via other 1200 series module

Safety and maintenance

Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

GLP features

Column-identification module for GLP

documentation of column type

Housing

All materials recyclable



Aglient 1200 series G1315B DAD

Wavelength range

190-950 nm

Light source

Deuterium and tungsten lamps

Detection type

1024 - element photodiode array

Short term noise (ASTM) single & mult-wavelength

± 1° x 10-5 AU at 254 and 750 nm

Drift

2° x 10-3 AU / hr at 254 nm

Linear absorbance range

> 2 AU (upper limit)

Wavelength accuracy

± 1 nm

Wavelength bunching

1 - 400 nm

Diode width

< 1 nm

Flow cells

Standard: 13µl volume 10 mm cell path length and 120 bar (1760 psi) pressure maximum
Semi-Micro: 5µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
Micro: 2µl volume, 3 mm cell path length and 120 bar (1760 psi) pressure maximum
High Pressure: 1.7µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure max.
80 nano: 0.08µl volume, 100 mm cell path length and 50 bar (725 psi) pressure max.
500 nano: 0.5µl volume, 10 mm cell path length and 50 bar (725 psi) pressure max.

Analog outputs

Recorder / integrator: 10 mV or 1 V, output range 
0.001 - 2 AU, two outputs

Control and data evaluation

Agilent Chemstation for LC

Slit width

1, 2, 4, 8, 16 nm

Communication types

CAN, GBIP, RS-232C, APG, Remote: ready, start, stop and shut down

Wavelength range

190-950 nm

Light source

Deuterium and tungsten lamps

Detection type

1024 - element photodiode array

Short term noise (ASTM) single & mult-wavelength

± 1° x 10-5 AU at 254 and 750 nm

Drift

2° x 10-3 AU / hr at 254 nm

Linear absorbance range

> 2 AU (upper limit)




















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